AutoTrader Web will not work for Kite accounts which have 2Factor TOTP enabled. The reason is obvious as the there is no way AutoTrader Web can get access to TOTP.
Hence it is recommended to keep it disabled, if you want to do automated trading. Process is given below:
Login to Kite with Web> Account > My profile > Password & Security. Under 2Factor TOTP, click on disable. Enter your password and PIN. And you are done.